Hit confirmation with CETSA
Hit confirmation service is based on the Cellular Thermal Shift Assay (CETSA) and allows for assessment of compound binding to a selected target protein in its native state without any modifications to the protein or the compound.
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In opposition to other target engagement assays performed on purified recombinant proteins or cellular assays relying on engineered systems, our service is based on CETSA provides a direct measurement of compound binding in a physiologically relevant manner (i.e. within the complex natural environment of lysates or intact cells) without modifying the compound or the target protein.
As it measures drug-target binding directly, the assay can be easily applied on different cell types, allowing for rapid translation of TE between sample matrices.
The label-free measure of target engagement means that CETSA effects are true reflection of the direct binding of the compound to the target in the natural environment of cells and tissues. Moreover, it is rapid to set up compared to engineered assay systems, which involve more time-consuming assay development.
With our CETSA-based hit confirmation service, you can benefit from:
- Cellular target engagement measurement, label-free and in a physiologically relevant system
- Rapid assay development, even for challenging targets
- Actionable data that enables you to confirm hits and prioritize compounds early in the project
Our hit confirmation service can be applied in lead generation for rapid assessment of cellular target engagement.
