HSV-2 IgM
High Quality Assays with Reproducible and Reliable Results
The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our Product Directory.
An enzyme immunoassay for the detection of IgM antibodies to herpes simplex virus type 2 (HSV-2) in serum. Herpes Simplex Virus is a common pathogen and its primary infection is usually asymptomatic. There are two immunologically distinct types of HSV: Type 1 and Type 2. HSV-1 isgenerally associated with oral infection and lesions above the waist, and HSV-2 is associated with genital infections and lesions below the waist. Clinical cases primarily are (1) eczema herpeticum with eczematous skin changes with numerous lesions, (2) gingivo-stomatitis, and (3) herpes sepsis, almost only found in premature newborn infants. The BioCheck HSV-2 IgM ELISA is an accurate serologic method to detect HSV-2 IgM specific-antibody in serum samples.Purified HSV-2 antigen is coated on the surface of microwells. Diluted patient serum is added to the wells, and the HSV-2 IgM- specific antibody, if present, binds to the antigen. All unbound materials are washed away. HRP-conjugate is added, which binds to the antibody-antigen complex. Excess HRP-conjugate is washed off and a solution of TMB Reagent is added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of HSV-2 IgM- specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls.
