Human Cytokine Array C4000

Assay Format

Number of Targets Detected

274

Species Detected

Human

Compatible Sample Types

Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates

Design Principle

Sandwich-based

Method of Detection

Chemiluminescence

Quantitative/Semi-Quantitative

Semi-Quantitative

Solid Support

Membrane

Target Names

Angiogenin, BDNF, BLC, BMP-4, BMP-6, Ck beta 8-1, CNTF, EGF, Eotaxin-1, Eotaxin-2, Eotaxin-3, FGF-6, FGF-7, Flt-3 Ligand, Fractalkine, GCP-2, GDNF, GM-CSF, I-309, IFN-gamma, IGFBP-1, IGFBP-2, IGFBP-4, IGF-1, IL-10, IL-13, IL-15, IL-16, IL-1 alpha, IL-1 beta, IL-1 ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, Leptin, Light, MCP-1, MCP-2, MCP-3, MCP-4, M-CSF, MDC, MIG, MIP-1 delta, MIP-3 alpha, NAP-2, NT-3, PARC, PDGF-BB, RANTES, SCF, SDF-1 alpha, TARC, TGF beta 1, TGF beta 3, TNF alpha, TNF beta, Adiponectin, AgRP, Angiopoietin-2, Amphiregulin, Axl, bFGF, beta-NGF, Betacellulin, CCL28, CTACK, Dtk, EGFR, ENA-78, Fas, FGF-4, FGF-9, GCSF, GITR Ligand, GITR, GRO alpha/beta/gamma, GRO alpha, HCC-4, HGF, ICAM-1, ICAM-3, IGFBP-3, IGFBP-6, IGF-1 R, IL-1 R4, IL-1 R1, IL-11, IL-12 p40, IL-12 p70, IL-17A, IL-2 R alpha, IL-6 R, IL-8, I-TAC, Lymphotactin, MIF, MIP-1 alpha, MIP-1 beta, MIP-3 beta, MSP alpha/beta, NT-4, Osteoprotegerin, Oncostatin M, PLGF, gp130, TNF RII, TNF RI, TECK, TIMP-1, TIMP-2, Thrombopoietin, TRAIL R3, TRAIL R4, uPAR, VEGF-A, VEGF-D, Activin A, ALCAM, CD80, BMP-5, BMP-7, Cardiotrophin-1, CD14, CXCL16, DR6, Endoglin, ErbB3, E-Selectin, Fas Ligand, ICAM-2, IGF-2, IL-1 R2, IL-10 R beta, IL-13 R alpha 2, IL-18 BP alpha, IL-18 R beta, MMP-3, IL-2 R beta, IL-2 R gamma, IL-21 R, IL-5 R alpha, IL-9, IP-10, LAP/TGF beta 1, Leptin R, LIF, L-Selectin, M-CSF R, MMP-1, MMP-13, MMP-9, MPIF-1, NGFR, PDGF-AA, PDGF-AB, PDGF R alpha, PDGF R beta, PECAM-1, Prolactin, SCF R, SDF-1 beta, Siglec-5, TGF alpha, TGF beta 2, Tie-1, Tie-2, TIMP-4, VE-Cadherin, VEGFR2, VEGFR3, Adipsin, BCAM, CD30, CD40, Fc gamma RIIB/C, Ferritin, FLRG, Follistatin, Furin, Galectin-7, GDF-15, Growth Hormone, IL-10 R alpha, IL-22, IL-28A, IL-29, IL-31, Insulin, Luteinizing hormone, LIMPII, LYVE-1, Marapsin, MICA, MICB, MMP-2, MMP-7, MMP-8, MMP-10, NCAM-1, Nidogen-1, Nr-CAM, NRG1-beta 1, Osteopontin, PAI-1, Platelet factor 4, PSA-total, RAGE, RANK, Resistin, SAA, Siglec-9, TACE, TIM-1, TRAIL R2, Trappin-2, TREM-1, TSH, TSLP, VCAM-1, VEGF-C, XEDAR, 4-1BB, ACE-2, Alpha-fetoprotein, Angiopoietin-1, Angiostatin, ANGPTL4, Beta-2 Microglobulin, BCMA, beta IG-H3, CA125, CA15-3, CA19-9, CA9, Cathepsin S, HCC-1, 6Ckine, CD23, CD40 Ligand, CEA, CEACAM-1, Cripto-1, CRP, DAN, Decorin, DKK-1, Dkk-3, Dkk-4, CD26, E-Cadherin, EDA-A2, EG-VEGF, TROP1, ErbB2, Erythropoietin R, FSH, HB-EGF, hCG intact, HVEM, IL-13 R alpha 1, IL-17B, IL-17C, IL-17F, IL-17 RA, Procalcitonin, PSA-free, S100 B, Sonic Hedgehog N-Terminal, Thyroglobulin, Ubiquitin+1

Product Features

• Easy to use
• No specialized equipment needed
• Compatible with nearly any liquid sample
• Proven technology (many publications)
• Highly sensitive (pg/ml)
• Sandwich ELISA specificity
• Higher density than ELISA, Western blot or bead-based multiplex

Suggested Applications

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Storage

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.