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Human/Mouse AKT Pathway Phosphorylation Array C1

RayBio® C-Series Human/Mouse AKT Pathway Phosphorylation Array C1. Detects 18 phosphorylated human and mouse proteins. Suitable for all liquid sample types but intended for use with cell and tissue lysates.

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RayBiotech Inc.

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Assay Format

Number of Targets Detected

18

Species Detected

Human, Mouse

Compatible Sample Types

Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates

Design Principle

Sandwich-based

Method of Detection

Chemiluminescence

Quantitative/Semi-Quantitative

Semi-Quantitative

Solid Support

Membrane

Target Names

Akt (P-Ser473) (PKB (P-Ser473)), AMPKa (P-Thr172), BAD (P-Ser112), 4E-BP1 (P-Thr36), ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187), GSK3a (P-Ser21), GSK3b (P-Ser9), mTOR (P-Ser2448), p27 (P-Thr198), P53 (P-Ser15), P70S6K (P-Thr421/Ser424), PDK1 (P-S241), PRAS40 (P-Thr246), PTEN (P-Ser380), Raf-1 (Ser301), RPS6 (P-Ser235/Ser236), RSK1 (P-Ser380), RSK2 (P-Ser386)

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Suggested Applications

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Storage

For best results, store the entire kit frozen a -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer a -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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