Human/Mouse AKT Pathway Phosphorylation Array C1
RayBio® C-Series Human/Mouse AKT Pathway Phosphorylation Array C1. Detects 18 phosphorylated human and mouse proteins. Suitable for all liquid sample types but intended for use with cell and tissue lysates.
Assay Format
Number of Targets Detected
18
Species Detected
Human, Mouse
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Design Principle
Sandwich-based
Method of Detection
Chemiluminescence
Quantitative/Semi-Quantitative
Semi-Quantitative
Solid Support
Membrane
Target Names
Akt (P-Ser473) (PKB (P-Ser473)), AMPKa (P-Thr172), BAD (P-Ser112), 4E-BP1 (P-Thr36), ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187), GSK3a (P-Ser21), GSK3b (P-Ser9), mTOR (P-Ser2448), p27 (P-Thr198), P53 (P-Ser15), P70S6K (P-Thr421/Ser424), PDK1 (P-S241), PRAS40 (P-Thr246), PTEN (P-Ser380), Raf-1 (Ser301), RPS6 (P-Ser235/Ser236), RSK1 (P-Ser380), RSK2 (P-Ser386)
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Suggested Applications
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage
For best results, store the entire kit frozen a -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer a -20°C and all other reagents undiluted at 4°C for no more than 3 months.