Human Phospho-H2AX (S139) and Total H2AX ELISA
RayBio® Human Phospho-H2AX (S139) and Total H2AX ELISA Kit. This assay semi-quantitatively measures phosphorylated H2AX (Ser139) and Total H2AX in lysate samples.
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Assay Format
Specificity
The antibody pair provided in this kit recognizes human H2AX phosphorylated at Serine-139 and total H2AX.
Species Detected
Human
Compatible Sample Types
Cell Lysates, Tissue Lysates
Design Principle
Sandwich-based
Method of Detection
Colorimetric
Quantitative/Semi-Quantitative
Semi-Quantitative
Solid Support
96-well Microplate
Protein Information
Accession Number
P16104
Gene ID
3014
Gene Symbols
H2AFX, H2AX
Protein Name & Synonyms
Histone H2AX (H2a/x) (Histone H2A.X)
Target Species
Human
Product Features
- Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
Storage
Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
