Human Phospho-H2AX (S139) and Total H2AX ELISA

Assay Format

Specificity

The antibody pair provided in this kit recognizes human H2AX phosphorylated at Serine-139 and total H2AX.

Species Detected

Human

Compatible Sample Types

Cell Lysates, Tissue Lysates

Design Principle

Sandwich-based

Method of Detection

Colorimetric

Quantitative/Semi-Quantitative

Semi-Quantitative

Solid Support

96-well Microplate

Protein Information

Accession Number

P16104

Gene ID

3014

Gene Symbols

H2AFX, H2AX

Protein Name & Synonyms

Histone H2AX (H2a/x) (Histone H2A.X)

Target Species

Human

Product Features

• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Storage

Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.