Human Phosphorylation Pathway Profiling Array C55
RayBio® C-Series Human Phosphorylation Pathway Profiling Array C55 Kit. Detects phosphorylated human proteins in 5 signaling pathways: MAPK, AKT, JAK/STAT, NF?B, and TGFß. Suitable for all liquid sample types but intended for use with cell and tissue lysates.
Place your order directly with the manufacturer.
Assay Format
Number of Targets Detected
55
Species Detected
Human
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Design Principle
Sandwich-based
Method of Detection
Chemiluminescence
Quantitative/Semi-Quantitative
Semi-Quantitative
Solid Support
Membrane
Target Names
4E-BP1 (P-Thr36), AKT (P-Ser473), AMPKa (P-Thr172), ATF2 (P-Thr69/71), ATM (P-Ser1981), BAD (P-Ser112), C-Fos (P-Thr232), c-Jun (P-Ser73), CREB (P-Ser133), EGFR (P-Ser1070), eIF-2a (P-Ser51), ERK1/2 (P-Thr202/P-Tyr204/P-Tyr185/P-Tyr187), GSK-3 alpha (P-Ser21), GSK-3 beta (P-Ser9), HDAC2 (P-Ser394), HDAC4 (P-Ser632), HSP27 (P-Ser82), IKBa (P-Ser32), JAK1 (P-Tyr1022), JAK2 (P-Tyr1007/1008), JNK (P-Thr183), MEK (P-Ser217/221), MKK3 (P-Ser189), MKK6 (P-Ser207), MSK1 (P-Ser376), MSK2 (P-Ser360), mTOR (P-Ser2448), NF-kB (P-Ser536), p27 (P-Thr198), p38 (P-Thr180/Tyr182), P53 (P-Ser15), P70S6K (P-Thr421/Ser424), PDK1 (P-Ser241), PRAS40 (P-Thr246), PTEN (P-Ser380), Raf-1 (P-Ser301), RPS6 (P-Ser235/236), RSK1 (P-Ser380), RSK2 (P-Ser386), SHP-1 (P-Ser591), SHP2 (P-Tyr542), SMAD1 (P-Ser463/465), SMAD2 (P-Ser245/250/255), SMAD4 (P-Thr277), SMAD5 (P-Ser463/465), Src (P-Tyr419), STAT1 (P-Ser727), STAT2 (P-Tyr689), STAT3 (P-Tyr705), STAT5 (P-Tyr694), STAT6 (P-Tyr641), TAK1 (P-Ser412), TBK1 (P-Ser172), TYK2 (P-Tyr1054), ZAP70 (P-Tyr292)
Suggested Applications
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
