Human TGF-beta 1 ELISA
RayBio® Human TGF beta 1 ELISA Kit for cell culture supernatants, plasma, and serum samples.
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Assay Format
Detection Range
18 pg/ml - 4000 pg/ml
Recommended Dilution (serum & plasma)
20 fold after treatment (see activation steps on page 6)
Sensitivity
18 pg/ml
Specificity
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human ANG, CD23, Eotaxin, GCSF, GM-CSF, GRO-alpha, GRO-beta, GRO-gamma, I-309, IFN-gamma, IL-1 alpha, IL-1 beta, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40), IL-12 (p70), IL-15, IL-16, IP-10, MCP-1, MCP-2, MCP-3, MCP-4, MCSF, MIG, MIP-1alpha, MIP-1beta, NAP-2, PDGF, PF-4, PARC, SCF, SDF-1alpha, TIMP-1, TIMP-2, TNF-alha, TGF-beta 2, TGF-beta 3, VEGF.
Species Detected
Human
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum
Design Principle
Sandwich-based
Method of Detection
Colorimetric
Quantitative/Semi-Quantitative
Quantitative
Solid Support
96-well Microplate
Protein Information
Accession Number
P01137
Gene ID
7040
Gene Symbols
TGFB1, TGFB
Protein Name & Synonyms
Transforming growth factor beta-1 (TGF-beta-1) [Cleaved into: Latency-associated peptide (LAP)]
Target Species
Human
Product Features
Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.
Storage
