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Human TGF-beta 1 ELISA

RayBio® Human TGF beta 1 ELISA Kit for cell culture supernatants, plasma, and serum samples.

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Assay Format

Detection Range

18 pg/ml - 4000 pg/ml

Recommended Dilution (serum & plasma)

20 fold after treatment (see activation steps on page 6)

Sensitivity

18 pg/ml

Specificity

This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human ANG, CD23, Eotaxin, GCSF, GM-CSF, GRO-alpha, GRO-beta, GRO-gamma, I-309, IFN-gamma, IL-1 alpha, IL-1 beta, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40), IL-12 (p70), IL-15, IL-16, IP-10, MCP-1, MCP-2, MCP-3, MCP-4, MCSF, MIG, MIP-1alpha, MIP-1beta, NAP-2, PDGF, PF-4, PARC, SCF, SDF-1alpha, TIMP-1, TIMP-2, TNF-alha, TGF-beta 2, TGF-beta 3, VEGF.

Species Detected

Human

Compatible Sample Types

Cell Culture Supernatants, Plasma, Serum

Design Principle

Sandwich-based

Method of Detection

Colorimetric

Quantitative/Semi-Quantitative

Quantitative

Solid Support

96-well Microplate

Protein Information

Accession Number

P01137

Gene ID

7040

Gene Symbols

TGFB1, TGFB

Protein Name & Synonyms

Transforming growth factor beta-1 (TGF-beta-1) [Cleaved into: Latency-associated peptide (LAP)]

Target Species

Human

Product Features

Prepare all reagents, samples and standards as instructed in the manual.

Add 100 µl of standard or sample to each well.

Incubate 2.5 h at RT or O/N at 4°C.

Add 100 µl of prepared biotin antibody to each well.

Incubate 1 h at RT.

Add 100 µl of prepared Streptavidin solution to each well.

Incubate 45 min at RT.

Add 100 µl of TMB One-Step Substrate Reagent to each well.

Incubate 30 min at RT.

Add 50 µl of Stop Solution to each well.

Read at 450 nm immediately.

Storage

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