HypoMethylCollector™
Novel technology that allows for positive identification of unmethylated DNA fragments
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The HypoMethylCollector™ Kit provides an efficient method for isolating non-methylated CpG dinucleotides from limited amounts of cell or tissue DNA.
Most CpG island enrichment methods require clusters of methylated CpGs. Since CpG islands are normally unmethylated isolation and evaluation of many human promoters contained within CpG islands has been difficult. The HypoMethylCollector™ Kit resolves this issue by utilizing a His-tagged recombinant CXXC protein domain from mouse Mbd1 in order to specifically bind non-methylated CpGs. This novel technology allows for positive identification of hypomethylated promoters or can be used to confirm the negative results obtained by methyl-CpG capture methods, such as Active Motif's MethylCollector Ultra Kit. This simple and sensitive technique is suitable for use in many downstream applications, such s real time or endpoint PCR analysis of the methylation status of particular promoters in normal and diseased samples, rapid screening of the methylation status of multiple loci, sequencing, or amplificaiton and labeling for microarray analysis. It can also be used to detect changes in DNA methylation in other situations, including normal cellular differentiation and aging.
