in vitro Comet Assay by Contract Service
Sensitive DNA damage assessment Very low compound requirement Rapid turnaround, ideal for screening Reliable testing by genetic toxicology experts Assay Principles The Comet assay, also called single cell gel electrophoresis, is a technique used for the sensitive detection of DNA damage in individual cells following exposure to a test compound. Electrophoresis is performed on exposed cells that have been embedded i…
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- Sensitive DNA damage assessment
- Very low compound requirement
- Rapid turnaround, ideal for screening
- Reliable testing by genetic toxicology experts
- Assay Principles
The Comet assay, also called single cell gel electrophoresis, is a technique used for the sensitive detection of DNA damage in individual cells following exposure to a test compound. Electrophoresis is performed on exposed cells that have been embedded in agarose microgels. Damage resulting in DNA fragmentation is detectable by migration away from the nucleus. A DNA-stained damaged cell reveals the characteristic comet-like appearance: a bright ‘head’ (nucleus) and a ‘tail’ streak (damaged DNA). Types of damage measured include DNA strand breakage, DNA adducts, oxidative damage and cross-links. The length of the comet ‘tail’ and the intensity of DNA staining within it are proportional to the extent of DNA damage.