Influenza Virus A IgM
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The Influenza Virus A IgM-ELISA is intended for the qualitative determination of IgM class antibodies to Influenza virus A in human serum or plasma (citrate).The qualitative immunoenzymatic determination of IgM-class antibodies to Influenza Virus A is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter strip wells are precoated with Influenza Virus A antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-human IgM conjugate is added. This conjugate binds to the captured Influenza Virus A specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of Influenza Virus A specific IgM antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.
