Invitrogen™ LIVE/DEAD™ Fixable Far Red Dead Cell Stain Kit
The LIVE/DEAD Fixable Far Red Dead Cell Stain Kit provides the stable components to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. The amine-reactive LIVE/DEAD Fixable Far Red Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines in cel…
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The LIVE/DEAD Fixable Far Red Dead Cell Stain Kit provides the stable components to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. The amine-reactive LIVE/DEAD Fixable Far Red Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines in cells with compromised membranes, whereas this reactivity is restricted to amines on the cell surface in viable cells. The fluorescence difference between dead and live cells is greater than 50-fold. The staining pattern is preserved following formaldehyde fixation.
Features
- Stable—dyes are freeze dried in separate vials to maintain stability
- Robust—staining pattern is the same before and after fixation
- Bright signal—allows for easy distinction between live/dead cells in single channel
The LIVE/DEAD Fixable Far Red Stain can be excited using either a red laser. The far-red fluorescent dye has an excitation maximum of approximately 633 nm that is excited well with a red or HeNe laser, and an emission of approximately 655 nm. Since live and dead cells can be discriminated using a single dye and a single channel of a flow cytometer, it is an ideal choice for multi-color experiments.
