Invitrogen™ LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit
The LIVE/DEAD Fixable Violet Dead Cell Stain Kit provides the stable components to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. The amine-reactive LIVE/DEAD Fixable Violet Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines in cells…
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The LIVE/DEAD Fixable Violet Dead Cell Stain Kit provides the stable components to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. The amine-reactive LIVE/DEAD Fixable Violet Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines in cells with compromised membranes, whereas this reactivity is restricted to amines on the cell surface in viable cells. The fluorescence difference between dead and live cells is greater than 50-fold. The staining pattern is preserved following formaldehyde fixation.
Features
- Stable—dyes are freeze dried in separate vials to maintain stability
- Robust—staining pattern is the same before and after fixation
- Low compensation—minimal spectral overlap between other fluorophores
The LIVE/DEAD Fixable Violet Stain minimizes compensation between other violet dyes and dyes that that excite using a 488-nm blue laser. The LIVE/DEAD Fixable Violet Stain has an excitation maximum of approximately 416 nm, and an emission of approximately 451 nm, allowing for capture in the first channel of most violet light laser flow cytometers.
