Invitrogen™ LIVE/DEAD™ FungaLight™ Yeast Viability Kit
The LIVE/DEAD FungaLight Yeast Viability Kit enables researchers to easily, reliably and quantitatively distinguish live and dead yeast in minutes by flow cytometry. The kit contains solutions of the SYTO™ 9 green-fluorescent nucleic acid stain and the red-fluorescent nucleic acid stain, propidium iodide. SYTO 9 stain generally labels all yeast in a population, with or without intact membranes. Propidium iodide penetrates only…
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The LIVE/DEAD FungaLight Yeast Viability Kit enables researchers to easily, reliably and quantitatively distinguish live and dead yeast in minutes by flow cytometry. The kit contains solutions of the SYTO™ 9 green-fluorescent nucleic acid stain and the red-fluorescent nucleic acid stain, propidium iodide. SYTO 9 stain generally labels all yeast in a population, with or without intact membranes. Propidium iodide penetrates only yeast with damaged membranes, causing a reduction in the SYTO 9 stain fluorescence by fluorescence resonance energy transfer (FRET) when both dyes are present. As a result, yeast with intact membranes stain fluorescent green, whereas yeast with damaged membranes stain fluorescent red.
