Invitrogen™ pHrodo™ Red, succinimidyl ester (SE)
Get faster staining and more accurate results - without the need for wash steps or quencher dye
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Great product to use for phagocytosis assays!
Fluorescent microscopy of internalized bacteria
I use this product in a project in which we are characterizing an immune cell's ability to phagocytose bacteria. This product is wonderful and will light up red fairly quickly once the bacteria has been internalized into an acidic compartment within the cell. This product is super easy to use, and it only takes about 10-20 minutes for staining and washing before the bacteria can be added for infection.
Review Date: 22 Nov 2019 | Thermo Fisher Scientific
New pH-sensitive pHrodo™ Red dye conjugates give faster and accurate results for phagocytosis assays.
pHrodo™ Red dye conjugates are non-fluorescent outside the cell but fluoresce brightly red in phagosomes
- Specific detection of phagocytosis and endocytosis
- Reduced signal variability and improved timing in sensitive experiments
- Multiplex with green dyes such as GFP, Fluo-4, or calcein
The fluorescence of the novel pHrodo™ Red dye dramatically increases as pH decreases from neutral to the acidic, making it an ideal tool to study phagocytosis and its regulation by drugs and/or environmental factors. The lack of fluorescence outside the cell eliminates the need for wash steps and quencher dyes.
Use the ready-made pHrodo™ Red E.coli BioParticles® conjugates in imaging or flow applications, or pHrodo™ Red SE, the activated succinimidyl ester, for labeling microorganisms or proteins of your choice.
For Research Use Only. Not for use in diagnostic procedures
