iScript™ RT-qPCR Sample Preparation Reagent, 100 reactions
100 reactions, 10 ml, contains100 reactions, 10 ml, contains RNA stabilizers
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Good value for experiments
transcripomics
This kit contains all reagents to perform cDNA libraries in an affordable and reproducible format. You can re-order and the materials will arrive super fast, making lab work more efficient
Review Date: 12 Jun 2023 | Bio-Rad
Packaging Options
- Catalog #170-8898, 100 reactions, 10 ml, contains RNase inhibitors and RNA stabilizers
- Catalog # 170-8899 , 500 reactions, 5 x 10 ml, contains RNase inhibitors and RNA stabilizers
Description
iScript™ RT-qPCR sample preparation reagent provides a simple and rapid procedure (<10 min) for isolating total RNA from cultured cells to use for RT-qPCR. This reagent delivers efficient cell lysis and RNA stabilization for sensitive qPCR without traditional RNA sample preparation.
Key Features and Benefits
- Have ease-of-use and simplicity — one-buffer formulation contains all of the necessary components to perform cell lysis and RNA stabilization in 5–10 min
- Benefit from superior compatibility with qPCR directly from cells — sensitive detection of high-, medium-, and low-copy gene targets directly from cell lysates using real-time qPCR when combined with iScript reverse transcription and qPCR reagents
- Maximize data from very few cells — reagent enables multiplex real-time detection of up to 4 targets from as few as 10 cells
- Extend isolated RNA shelf-life — isolated RNA is stable for up to 6 months when frozen
- Use with different cell types — compatible with both suspension and adherent cell cultures
