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Vericheck ddPCR Empty-Full Capsid Kit

Quickly measure empty-full ratio with one assay to produce AAV vectors

Bio-Rad

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The Vericheck ddPCR Empty-Full Capsid Kit enables a quick and precise measurement of the empty-full capsid ratio that is needed to produce AAV vectors. The empty-full capsid ratio is a Critical Quality Attribute (CQA) in AAV vector production since high amounts of empty capsids may cause unwanted immunotoxicity. This kit simultaneously provides consistent and reliable capsid content measurements across the AAV production process with high precision, multi-sample type compatibility, low-sample input, fast turnaround time, and easy data analysis.

Features & Benefits

  • Sample type compatibility — crude lysate and purified
  • Low sample input — only 106 virus particles required to get results
  • Fast turnaround time — up to 14 samples per plate in less than 1 day
  • High precision — coefficient of variation (CV) less than 5%
  • Simplified data analysis – streamlined process providing simultaneous results for genome titer, capsid titer, and empty-full ratio

Applications & Uses

  • Simply and precisely measure empty-full ratio with one assay
  • Optimized to provide precision throughout the AAV characterization process

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