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iTaq™ Universal SYBR® Green One-Step Kit, 500 x 20 µl rxns, 5 ml (5 x 1 ml)

Bio-Rad1725151

5 ml (5 x 1 ml), one-step RT-PCR kit, includes 2x one-step SYBR Green reaction mix (Taq polymerase, buffers, ROX normalization dyes), iScript reverse transcriptase, and nuclease-free water, for 500 x 20 µl reactions

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Our Universal Real-Time PCR Reagents Web App brings you in-depth product information, individual and competitive performance data, interactive tutorials, 3-D animated videos, technical manuals, and more.

Use our combined iScript™ RNase H+reverse transcriptase and antibody hot-start iTaq DNA polymerase kit formulation to complete your SYBR® Green real-time reactions in one step — PCR efficiency is improved over a wide dynamic range and under various conditions.

  • Advanced formulation for amplifying difficult targets and challenging samples
  • Can be combined with our PrimePCR™ assays for a simplified workflow
  • Universal — works on any real-time PCR system

Kit Components

  • Reverse transcriptase – 125 µl
  • Reaction mix – 5 ml (5 x 1 ml)

Instrument Compatibility

iTaq Universal SYBR Green One-Step RT-qPCR Kit is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:

* BSA must be added according to instrument specifications

Applications and Uses of the iTaq® Universal SYBR® Green One-Step Kit

  • qPCR/real-time PCR
  • Gene expression analysis
  • Absolute quantification
  • Mutation detection
  • Pathogen detection
  • Viral detection (load)
  • Genetically modified organism (GMO) characterization
  • Genetic profiling

The iTaq™ universal SYBR® Green one-step kit is also available in a format of 100 reactions (171-5150) .

Related Products

Bio-Rad has also developed one-step kits for probe-specific applications .

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