iTaq™ Universal SYBR® Green One-Step Kit, 500 x 20 µl rxns, 5 ml (5 x 1 ml)
5 ml (5 x 1 ml), one-step RT-PCR kit, includes 2x one-step SYBR Green reaction mix (Taq polymerase, buffers, ROX normalization dyes), iScript reverse transcriptase, and nuclease-free water, for 500 x 20 µl reactions
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Use our combined iScript™ RNase H+reverse transcriptase and antibody hot-start iTaq DNA polymerase kit formulation to complete your SYBR® Green real-time reactions in one step — PCR efficiency is improved over a wide dynamic range and under various conditions.
- Advanced formulation for amplifying difficult targets and challenging samples
- Can be combined with our PrimePCR™ assays for a simplified workflow
- Universal — works on any real-time PCR system
Kit Components
- Reverse transcriptase – 125 µl
- Reaction mix – 5 ml (5 x 1 ml)
Instrument Compatibility
iTaq Universal SYBR Green One-Step RT-qPCR Kit is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:
* BSA must be added according to instrument specifications
Applications and Uses of the iTaq® Universal SYBR® Green One-Step Kit
- qPCR/real-time PCR
- Gene expression analysis
- Absolute quantification
- Mutation detection
- Pathogen detection
- Viral detection (load)
- Genetically modified organism (GMO) characterization
- Genetic profiling
The iTaq™ universal SYBR® Green one-step kit is also available in a format of 100 reactions (171-5150) .
Related Products
Bio-Rad has also developed one-step kits for probe-specific applications .
