KAPA Blood PCR Kits

KAPA Blood DNA Polymerase is a second-generation enzyme derived through a process of directed evolution, and is the first DNA polymerase engineered specifically for the amplification of DNA directly from whole blood.

DNA fragments may be amplified directly from reactions containing 1%-20% (v/v) whole human blood without pretreatment of blood samples or DNA isolation, significantly reducing contamination risk, turnaround time, and the cost of genetic testing.

Features:

Reduce contamination risk, turnaround time, and cost

• Amplify DNA fragments directly from blood, minimizing the risk of sample cross-contamination
• Reduce the turnaround time and cost of genetic testing

Compatibility with existing workflows

• Integrate with existing protocols and detection methods using optimized, convenient 2X PCR Mixes:
  - Blood PCR Mix A for fluorescent detection systems
  - Blood PCR Mix B for endpoint PCR and detection of GC-rich targets

Direct amplification of non-human blood

• Direct amplification of DNA from the blood of non-human species, such as other mammals and birds
• Reduces cost and turnaround time in PCR-based veterinary testing

Applications:

• Genetic testing
• Amplicons with a GC content <65% up to 3.5 kb in length; GC content >65% up to 1 kb
• Single amplicon or Multiplex PCR assays, using unlabeled or fluorescently-labeled primers
• Routinely-used detection methods
• Paternity testing using the PowerPlex® 16 System