KAPA Library Preparation Kits for Ion Torrent

KAPA DNA Library Preparation Kits for Ion Torrent sequencing platforms contain evolved and optimally formulated enzymes that enable the highest overall coverage from the least amount of total sequencing.

Kits are optimized to achieve significantly higher library yields and contain KAPA HiFi for high-fidelity, high-efficiency and low bias library amplification. This ensures the highest library complexity and sequence data quality, particularly from low input and difficult samples.

Features:

Achieve greater library complexity

• Higher yields of adapter-ligated library translates to higher library complexity
• Fewer cycles of amplification are needed, which results in lower duplication rates
• PCR-free workflows possible from inputs ≥100 ng

Reduced amplification bias and improve coverage

• KAPA HiFi DNA Polymerase is the gold standard enzyme for high-yield, low-bias library amplification, with a 40X lower error rate than Platinum® Taq HiFi
• KAPA HiFi reduces PCR bias and improves coverage uniformity of GC- and AT-rich regions, promoters, low complexity, and other challenging regions

Multiplex with confidence

• 24 barcoded adapters available in sets of 8
• Stringent quality-controlled adapters with <0.05% cross contamination

Automation compatible configuration

• Plate-friendly reaction volumes and robot-friendly pipetting volumes to accommodate the requirements for liquid handling

Applications:

• Whole Genome Sequencing
• ChIP-Seq
• Whole Exome Sequencing
• Amplicon sequencing
• Sequencing
• Targeted Sequencing (Capture)
• Methyl-Seq