KAPA Library Quantification Kits

KAPA Library Quantification Kits contain all the reagents needed for the accurate, reliable and reproducible qPCR-based quantification of NGS libraries prior to pooling for capture or flow cell amplification.

Kits contain KAPA SYBR® FAST qPCR Master Mix, optimized for high-performance SYBR Green I-based qPCR. The engineered KAPA SYBR FAST DNA Polymerase contained in the Master Mix amplifies GC- and AT-rich DNA fragments of different lengths with similar efficiency, making it the only qPCR Master Mix capable of accurate qPCR-based quantification of NGS libraries. The pre-diluted set of DNA Standards contained in each kit is carefully quality-controlled to ensure lot-to-lot consistency and avoid data drift over time. Optimized Primer Premixes ensure optimal PCR efficiency.

Features:

Accurately quantify PCR-competent sequencing templates

• qPCR only “counts” those library molecules that can be sequenced
• More consistent cluster densities or template-to-bead ratios enable optimal utilization of sequencing resources

Improve pooling for multiplexed sequencing

• Library concentrations determined by qPCR are more reliable compared to other methods
• The engineered KAPA SYBR FAST qPCR Master Mix enables accurate pooling of libraries with extreme GC-contents or longer inserts

Eliminate drift with carefully quality-controlled DNA Standards

• Prediluted DNA Standards eliminate quantification errors associated with preparation of standard curves using in-house standards
• KAPA DNA Standards undergo strict quality control to ensure lot-to-lot consistency and eliminate data drift over time

Improve throughput with automation

• Library quantification assay is compatible with 96- and 384-well format
• Library dilution, reaction setup and data analysis can be automated for HTP pipelines

Applications:

• Quantification of libraries prior to pooling for multiplexed sequencing
• Quantification of individual libraries or library pools prior to cluster amplification or emPCR
• Quantification of libraries prior to and after hybridization capture
• Quality control, optimization and troubleshooting of library construction processes or workflows