KAPA SYBR FAST One-Step qRT-PCR Kits
KAPA SYBR FAST One-Step qRT-PCR Kits are designed for optimal performance in stringent qRT-PCR reaction conditions, exhibiting dramatic improvements in sensitivity, specificity, reaction efficiency, and fluorescence. The kit is optimized for rapid one-step, one-tube RNA quantification, reducing experimental variation and contamination with a convenient qRT-PCR protocol. KAPA SYBR FAST One-Step qRT-PCR Kits contain M-MuLV r…
Fluorescence and optimal reaction efficiencies
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KAPA SYBR FAST One-Step qRT-PCR Kits are designed for optimal performance in stringent qRT-PCR reaction conditions, exhibiting dramatic improvements in sensitivity, specificity, reaction efficiency, and fluorescence.
The kit is optimized for rapid one-step, one-tube RNA quantification, reducing experimental variation and contamination with a convenient qRT-PCR protocol. KAPA SYBR FAST One-Step qRT-PCR Kits contain M-MuLV reverse transcriptase, RNase inhibitor, and a novel DNA polymerase evolved via directed evolution.
Features:
Increase signal and reaction efficiency
- Consistently higher amplification efficiencies for accurate qPCR
- Higher fluorescence, earlier Cq values and improved reaction efficiencies
Amplify across a broad range of target lengths
- Robust performance independent of amplicon size
Improve sensitivity and reproducibility
- Accurate interrogation across a wide range of RNA template concentrations
Applications:
- Gene expression analysis
- Low-copy gene detection
- Microarray validation
- miRNA research
- Gene knockdown validation
