KAPA SYBR® FAST qPCR Kits
KAPA SYBR FAST qPCR Kits contain the first DNA polymerase engineered via directed evolution to be more tolerant of SYBR Green I dye inhibition. The improved robustness, processivity, and speed of KAPA SYBR FAST qPCR Kits result in consistently high amplification efficiencies enabling more accurate relative quantification for gene expression analysis. KAPA SYBR FAST qPCR Kits, developed to perform optimally in stringent rea…
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KAPA SYBR FAST qPCR Kits contain the first DNA polymerase engineered via directed evolution to be more tolerant of SYBR Green I dye inhibition.
The improved robustness, processivity, and speed of KAPA SYBR FAST qPCR Kits result in consistently high amplification efficiencies enabling more accurate relative quantification for gene expression analysis. KAPA SYBR FAST qPCR Kits, developed to perform optimally in stringent real-time PCR reaction conditions, exhibit dramatic improvements to signal-to-noise ratio (fluorescence), quantification cycle (Cq), linearity, and sensitivity.
Quantitate changes in gene expression more accurately
- High reaction efficiency between 95–105% improves accuracy and reproducibility
- Unbiased efficiency across a wide range of GC contents and amplicon lengths
Detect low copy and difficult targets consistently
- Improved processivity results in earlier Cq scores
- Higher fluorescence detection across varying AT- and GC-rich targets
- Novel enzyme is resistant to SYBR inhibition
Complete real-time PCR runs in just 40 minutes
- 55% shorter run times with fast cycling protocol
- Maintain high performance when switching from standard to fast protocols
Applications:
- Gene expression
- Gene knockdown validation
- Microarray validation
- ChIP analysis
- Low copy detection
- Absolute quantification of NGS libraries
