KAPA3G Plant PCR Kits

KAPA3G Plant PCR Kits are designed for PCR of plant-derived DNA, using either purified DNA or DNA prepared by crude extraction methods (crude sample PCR).

In addition, the KAPA3G Plant PCR Kit can be used to amplify DNA from plant material added directly to the PCR (direct PCR). The KAPA3G Plant PCR Kit contains KAPA3G DNA Polymerase, a novel, third-generation (3G) enzyme which was engineered via a process of directed evolution for improved tolerance to common plant-derived PCR inhibitors such as polyphenolics and polysaccharides.

The KAPA3G Plant PCR Kits are optimized for fast and efficient amplification of plant DNA from crude samples, DNA-containing carry-over inhibitors from crude extraction methods, and purified DNA.

Features:

Amplify long targets from crude samples and purified DNA

• Amplify fragments up to 5 kb from purified DNA and crude samples
• High yield and specificity with purified DNA and crude samples

Perform direct PCR from a variety of plant species and tissue types

• Direct PCR with leaf disc or seed as template
• No need for time-consuming DNA extractions

Streamline workflows and improve turnaround times

• Perform PCR in half the time compared with wild-type enzymes
• Eliminate the need for time-consuming DNA extractions

Improve success rates with novel crude sample plant PCR workflow

• Use extraction buffer to prepare crude extracts for plant PCR in just 5 minutes
• High success rates with even the most challenging sample types

Applications:

• Amplification of fragments up to 5 kb in size from purified plant DNA, extracted with commercial kits or CTAB-based methods
• Direct PCR from leaf discs, seed samples, and other plant tissue types
• PCR from crude plant DNA extracts, prepared from leaf and/or seed material