KOD HiFi DNA Polymerase

Pure recombinant high-fidelity DNA polymerase from Thermococcus kodakaraensis KOD1

KOD HiFi DNA Polymerase* is a recombinant form of Thermococcus kodakaraensis KOD1 DNA polymerase. KOD HiFi is a high fidelity thermostable polymerase that amplifies target DNA up to 6 kbp in length with superior accuracy and yield. The enzyme’s 3′→5′ exonuclease-dependent proofreading activity results in a lower PCR mutation frequency than any other commercially available DNA polymerase. The elongation rate and processivity are 5 times and 10 to 15 times higher, respectively, than Pfu DNA polymerase, resulting in highly accurate and robust yield in a short reaction time. The enzyme generates blunt-ended PCR products suitable for cloning with Novagen’s Perfectly Blunt® and LIC Vector Kits.

Unit definition: one unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 minutes at 75°C, in a reaction containing 20 mM Tris-HCl (pH 7.5 at 25°C), 8 mM MgCl2, 0.5 mM DTT, 50 mg/ml BSA, 150 µM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]dTTP) and 150 µg/ml activated calf thymus DNA.

Features
• Higher fidelity than Pfu DNA polymerase—excellent for cloning
• Greater yield—extension speed is 2X faster than Taq DNA polymerase and 5X faster than Pfu DNA polymerase
• More accurate PCR in a shorter time
• Higher processivity—sequential nucleotide polymerization is 10- to 15-fold greater than Pfu and Tli DNA polymerases
• No truncated amplification products