KOD XL DNA Polymerase
High-performance enzyme blend for long and accurate PCRKOD XL DNA Polymerase* is an optimized blend of KOD HiFi DNA Polymerase and a mutant form of KOD HiFi that is deficient in 3′→5′ exonuclease activity. This enzyme mixture is designed for reliable amplification of long, complex targets with robust yield and high accuracy. It can also be used for incorporation of dITP, dUTP and derivatized dNTPs in PCR amplicons. KOD XL DNA…
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High-performance enzyme blend for long and accurate PCR
KOD XL DNA Polymerase* is an optimized blend of KOD HiFi DNA Polymerase and a mutant form of KOD HiFi that is deficient in 3′→5′ exonuclease activity. This enzyme mixture is designed for reliable amplification of long, complex targets with robust yield and high accuracy. It can also be used for incorporation of dITP, dUTP and derivatized dNTPs in PCR amplicons. KOD XL DNA Polymerase generates a mixture of PCR products with blunt and 3′-dA overhangs, suitable for cloning with Novagen’s Perfectly Blunt®, AccepTor™, and LIC Vector Kits.
Unit definition: one unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 minutes at 75°C, in a reaction containing 20 mM Tris-HCl (pH 7.5 at 25°C), 8 mM MgCl2, 0.5 mM DTT, 50 mg/ml BSA, 150 µM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]dTTP) and 150 µg/ml activated calf thymus DNA.
Features
• Ideal for amplification of large DNA fragments from purified DNA or crude samples
• Amplifies DNA templates up to 30 kbp
• Successfully amplifies GC-rich sequences
• Efficiently incorporates dITP, dUTP and derivatized dNTPs
