KRIBIOLISA™ Anti-Atezolizumab (TECENTRIQ) ELISA
Enzyme Immunoassay for the quantitative determination of Anti-Atezolizumab antibodies in serum and plasma.
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Principle:
The method employs the competitive enzyme immunoassay technique. Atezolizumab is pre-coated onto microwells. Samples or standards along with the tracer are pipetted into microwells and antibodies to Atezolizumab present in the standards or sample will compete with tracer and bound by the capture antibody. After washing microwells, HRP conjugate is pipetted and incubated. Free HRP conjugate will be removed by washing cycle. The ready to use substrate solution (TMB) is added to microwells and color develops inversely proportionally to the amount of Anti-Atezolizumab present in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
- Calibrator Range: 250 ng/ml - 2500 ng/ml
- Sensitivity: 62.5 ng/ml
- Sample Type: human serum and plasma
- Reactivity: Human
- Shipping Condition: 2 - 8 Deg C
- Detection Method: Colorimetric
- Usage: For In-Vitro Diagnostic Use Only
- Research Area: Immune Checkpoints
Krishgen kits have over 1000 citations, with our ELISA kits being used for publications in 15+ countries.
