KRIBIOLISA™ Bovine Serum Albumin ELISA *
Immunoassay for the measurement of Bovine Serum Albumin in cell culture samples.
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Principle:
The method employs sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and Bovine Serum Albumin present in the sample are bound by the antibodies. Biotin labeled antibody is added and followed by Streptavidin-HRP is pipetted and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Bovine Serum Albumin in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
- Calibrator Range: 0 ng/ml - 30000 ng/ml
- Sensitivity: 161.2 ng/ml
- Sample Type: Cell Culture Supernatant and other Biological Preparations
- Reactivity: Bovine
- Shipping Condition: 2 - 8 Deg C
- Detection Method: Colorimetric
- Usage: For Research Use Only
- Sensitive measurement of contaminants
- Low intra-assay and inter-assay variability allows for streamlined testing High specific for the target analyte
- Lot to Lot consistency.
- Species Independent.
- Priced better than competitors
- Validated for various sample matrices
Krishgen kits have over 1000 citations, with our ELISA kits being used for publications in 15+ countries.
