KRIBIOLISA™ PEG-GCSF (Pegylated Filgrastim) ELISA
Enzyme Immunoassay for the quantitative determination of PEG-GCSF in serum, plasma and cell culture supernatant.
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PEG-GCSF (Pegylated Filgrastim) ELISA kit for the quantitative detection of PEG-GCSF in cell culture and other purified samples. Sensitive, specific and ready to use assay kits from Krishgen Biosystems.
Intended Use:
The KRIBIOLISA™ PEG-GCSF ELISA is used as an analytical tool for quantitative determination of PEG-GCSF in serum, plasma and cell culture supernatant.
Principle:
The method employs the quantitative sandwich enzyme immunoassay technique. Anti- PEG-GCSF antibody is pre-coated onto microwells. In the first step, samples and standards are pipetted into microwells. If PEG-GCSF i s present in samples or standards, it will form complex with Anti- PEG-GCSF antibody, which is pre-coated onto microwells. In the second step, Anti- PEG-GCSF Biotin conjugate is pipetted and incubated. In the third step, HRP Conjugate is pipetted and incubated. Free HRP conjugate will be removed by a washing step. In the fourth step, TMB substrate is added to microwells and color develops proportionally to the amount of PEG-GCSF present in samples or standards. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
- Calibrator Range: 0 ng/ml - 160 ng/ml
- Sensitivity: 5 ng/ml
- Sample Type: serum, plasma and cell culture supernatant
- Shipping Condition: 2 - 8 Deg C
- Detection Method: Colorimetric
- Usage: For Research Use Only
