KRIBIOLISA™ Vedolizumab (ENTYVIO) ELISA

The Vedolizumab (Entyvio) ELISA kit is intended for the quantitative determination of Vedolizumab (Entyvio) in serum, plasma and cell culture samples. RUO only.

About the Ustekinumab ELISA kit:

• Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity
• Recovery rates are 80 - 120%
• Validated as per US FDA guidelines for Bioassays
• Optimized for matrix effects to ensure higher sensitivity
• Uses the innovator drug as calibrator, and is optimized for biosimilars studies

Principle:

The method employs the quantitative sandwich enzyme immunoassay technique. Antibodies to Vedolizumab are pre-coated onto microwells. Samples and standards are pipetted into microwells and human Vedolizumab present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-Vedolizumab antibody is pipetted and incubated. After washing microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Vedolizumab in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

• Calibrator Range: 31.25 ng/ml - 2000 ng/ml
• Sensitivity: 28 ng/ml
• Sample Type: serum, plasma and cell culture supernatant
• Shipping Condition: 2 - 8 Deg C
• Detection Method: Colorimetric
• Usage: For Research Use Only