Lactose Assay Kit
Sufficient for 100 colorimetric or fluorometric tests.
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Great results within the reported acceptance criteria.
To measure lactose content in milk
Suitable for the direct measurement of lactose levels in various biological samples (serum, plasma, other body fluids, food, growth media, etc.).
Review Date: 13 Dec 2019 | Merck
Lactose is a disaccharide formed by the condensation of one galactose and one glucose molecule. Lactose is the major sugar in the milk of most species, typically present between 2–8%. The enzyme lactase hydrolyzes lactose to its constituent monosaccharides. In neonates, glucose released via the action of lactase is a major energy source.
In this assay, lactose concentration is determined by an enzymatic assay, in which lactose is converted to galactose and glucose. The galactose is subsequently oxidized, resulting in a colorimetric (570 nm)/ fluorometric (λex = 535 nm/λem = 587 nm) product, proportional to the lactose initially present. This kit is suitable for use with various biological samples and has a linear range of detection between 0.2–1.0 nmole lactose for the fluorometric assay and 2–10 nmoles lactose for the colorimetric assay.
