LightCycler® 480 Real-Time PCR System
The Roche LightCycler 480 System - Another breakthrough in real-time PCR analysis of gene expression and melting curve-based mutation analysis.The Roche LightCycler 480 RT-PCR System combines amazing speed with exceptional accuracy and will meet the needs of a broad range of scientific applications in genomics research including array validation, gene-knockdown studies, and SNP analysis.This Roche Light Cycler PCR supports all…
Very reliable and very god pcr
Molecular biology
Best stuff for qpcr in uc
Review Date: 12 Nov 2023
I love it!
Molecular diagnostics
Instrument and software are user friendly, easy to use.
Review Date: 19 Jul 2021
Reliable, sensitive, modertely-priced qPCR instrument.
qPCR
Very relable, accurate & sensitive instrument for QPCR.
Review Date: 14 May 2020
Fantastic end product.
cDNA synthesis, PCR amplification
I find the instrument easy to set up and use. I find the reagents to be of a very high standard and the end products with very high quality.
Review Date: 28 Nov 2018
It is a stable and good instrument for real time PCR
THE real time machine - forget the others
We also use it, alongside a BioRad CFX96 and an ABI 7500. Out of the three, I think the Roche has the best sensitivity, and it also allows you to buy a 384 well block that can be easily interchanged if you want to add high throughput capability. However, the software (or at least the version that we have) is not particularly quick, cannot be loaded on a personal computer, and doesn't have many options for ways to export the data. And when we were interested in expanding into using HRM, we had to cough up a lot of cash to get the upgraded software module which we haven't found to be particularly useful. In contrast, the BioRad CFX is our go-to machine for most 96 well applications. The machine is good, consistent, has never needed maintenance (even though it's often used 6x per day), and has faster ramp times. But the real boon is the software. The basic software is simple to use, can be loaded on everyone's computer for offline analysis, and offers even the difficult normalization options (like normalization to the geometric mean of several reference genes, or ways to normalize across several plates in a multi-plate experiment). It even offers plate wide melt calibration so it gives MUCH more consistent results for anything reliant on melt (like HRM). On balance, the Roche instrument doesn't seem anywhere near superior enough to the BioRad to justify the terrible software. I'd highly recommend taking a look at the BioRad CFX96. It's the one that gets the most use around here!
Review Date: 24 Mar 2016
Unmatchable results with LNA probes & an innovation for gene expression studies.
Mutation detection & gene quantification
LightCycler 480 II System is a highly innovative instrument which uses specific optics for having the entire plate analysis simultaneously unlike well-to-well scanning systems producing high errors. LNA probes is a patented technology and offers exceptional specificity. LightCycler 480 II System is industry gold standard in terms of accuracy, reproducibility and repeatability of its results.
Review Date: 16 Dec 2015
qPCR
It is much better value for the money than the comparable instrument from LifeTech and better field scientist support. It is very robust and easily integrated with automation.
Review Date: 12 Oct 2015
Analyze Gene and microRNA Expression
The Roche LightCycler 480 is a PCR instrument that uses either 96 or 384 well plates to carry out automatic measurements on gene expression (or microRNA expression). The instrument can be easily used. It uses melting temperatures to determine the quality and purity of the template. The machine can be serviced by Roche in Indianapolis, IN, and is reasonably priced. I am a fan of Roche and I recommend this product for accurate measurement of gene or micRNA expression.
Review Date: 6 Aug 2015
It combines amazing speed with exceptional accuracy which will provides the needs of a broad range of scientific applications in genomics research including array validation, gene-knockdown studies, and SNP analysis. Another benefit is the good reproducibility and accuracy from a quick working instrument. It is very easy to perform optimization experiments and the software is easy to use. moreover, The LightCycler 480 PCR System utilizes a novel block technology to allow the rapid heating and cooling required to complete a 40-cycle, 384-well quantitative PCR run in less than 40 minutes and leads to extraordinary well-to-well temperature homogeneity and maximized inter-well, inter-cycle reproducibility. Finally, we suggest this system because the compatibility for automated sample-preparation, automated loading and connectivity to laboratory information management systems (LIMS) for integrated workflow monitoring offer uncompromising adaptability for a perfect fit.
Review Date: 30 Jan 2015
Real-Time PCR
This is a good machine for real-time PCR.
Review Date: 5 Nov 2014
The Roche LightCycler 480 System - Another breakthrough in real-time PCR analysis of gene expression and melting curve-based mutation analysis.
The Roche LightCycler 480 RT-PCR System combines amazing speed with exceptional accuracy and will meet the needs of a broad range of scientific applications in genomics research including array validation, gene-knockdown studies, and SNP analysis.
This Roche Light Cycler PCR supports all current probe formats and applications and its built-in versatility allows adaptation to future technological advances in genomics research.
Customer benefits from the Roche LightCycler® 480 System:
- Speed – rapid temperature control for superb specificity and yield.
The LightCycler 480 PCR System utilizes a novel block technology to allow the rapid heating and cooling required to complete a 40-cycle, 384-well quantitative PCR run in less than 40 minutes and leads to extraordinary well-to-well temperature homogeneity and maximized inter-well, inter-cycle reproducibility. - Accuracy – unbiased conditions for unambiguous results
Special arrangement of optical components ensures the uniform collection of signals across the plate and makes analysis independent of sample position or differences in reaction volume. - Versatility – utmost flexibility for maximum efficiency
Five excitation and six emission filters of the Roche LightCycler allow you to work with the assay format of your choice, including SYBR Green I, hydrolysis probes, probes, probes, Molecular Beacons, and others. - High-performance optical system from the LightCycler 480
Fluorescence excitation by a high-intensity xenon lamp, and a specially developed signal-collection system, result in maximal sensitivity for any assay format in all channels.
Additional Features of the LightCycler 480 PCR System:
- Customizable modular system concept
Building on the principles of the well-established, performance-proven LightCycler® Software 4.0, the Roche LightCycler® 480 platform comes equipped with modules for run control, Tm calling, and absolute quantification. Further customize your system to your specific needs by adding separately offered advanced analysis modules. - Especially developed reagents
A tailor-made hot-start PCR enzyme master mix is available for each type of LightCycler® 480 application. Built on Roche Diagnostics’ performance proven FastStart technology, these reagents ensure optimal reproducibility and high dynamic ranges. - Perfect integration into laboratory workflow
Compatibility for automated sample-preparation, automated loading and connectivity to laboratory information management systems (LIMS) for integrated workflow monitoring offer uncompromising adaptability for a perfect fit.
