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LightSwitch™ Promoter Reporter GoClone™ Collection

For use with The LightSwitch™ Luciferase Assay System for analysis of promoter regulation

Active Motif

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The LightSwitch Luciferase Assay System and Promoter Reporter Collection are important tools for determining how a transcription factor regulates its target genes.

Researchers can monitor changes in any promoter's activity in response to variations in transcription factor function that may be modulated by techniques like over-expression, knock-down or treatment with a known agonist or antagonist. LightSwitch Promoter Reporter constructs are also useful for assessing the functional consequences of transcription factor binding detected through a ChIP-based experiment and for measuring the effects of mutagenizing a putative transcription factor binding motif.

Applications:

  • Monitoring changes in promoter activity in response to variations in transcription factor function.
  • Assessing the functional consequences of transcription factor binding detected through a ChIP-based experiment.
  • Measuring the effects of mutagenizing a putative transcription factor binding motif.
  • Verifying computational predictions and supplementing microarray or Next-Generation sequencing data.

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