MagNA Pure Compact Nucleic Acid Isolation Kit I
Prefilled reagents for the isolation of genomic DNA from mammalian whole blood or cultured cells, total nucleic acid from mammalian serum or plasma, or bacterial DNA (from samples of mammalian origin or bacterial cultures) using the MagNA Pure Compact Instrument (Only in combination with MagNA Pure Bacteria Lysis Buffer (Cat. No. 04 659 180 001)) Application For general laboratory use. The MagNA Pure Compact Nucleic Acid Isol…
Prefilled reagents for the isolation of genomic DNA from mammalian whole blood or cultured cells, total nucleic acid from mammalian serum or plasma, or bacterial DNA (from samples of mammalian origin or bacterial cultures) using the MagNA Pure Compact Instrument
(Only in combination with MagNA Pure Bacteria Lysis Buffer (Cat. No. 04 659 180 001))
Application
For general laboratory use. The MagNA Pure Compact Nucleic Acid Isolation Kit I is specifically designed to isolate highly purified genomic DNA from mammalian whole blood, blood cells, buffy coat, or cultured cells, and total nucleic acid (e.g., viral DNA and RNA) from mammalian serum or plasma, using the MagNA Pure Compact Instrument. In combination with the MagNA Pure Bacteria Lysis Buffer (Cat. No. 04 659 180 001) and the DNA_Bacteria purification protocol, bacterial DNA can be isolated from samples of mammalian origin (e.g., urine, bronchoalveolar lavage [BAL] sputum, cerebrospinal fluid [CSF], swabs) or bacterial cultures. The purified nucleic acid can be used directly in PCR or RT-PCR on the LightCycler® Carousel-Based Systems, the LightCycler® 480 System, or standard thermal block cyclers.
Note: The MagNA Pure Compact Nucleic Acid Isolation Kit I cannot be used for co-isolation of RNA from mammalian whole blood or cultured cells.
Benefits
- Isolate genomic DNA from mammalian whole blood, blood cells, buffy coat, or cultured cells.
- Isolate viral nucleic acid from serum or plasma.
- Isolate bacterial DNA from different sample types.
Product Description
Number of isolations/sample type: 32 isolations (4 × 8) from
- 100-400 µl mammalian whole blood (containing no more than 5 × 103 cells/µl)
- 100-400 µl suspension of mammalian blood cells (white blood cells [WBCs) or peripheral blood mononuclear cells [PBMCs] or buffy coat [containing no more than 5 × 103 cells/µl])
- 100-400 µl mammalian serum or plasma
- 100 µl suspension of cultured cells (containing no more than 1 × 106 cells)
If combined with MagNA Pure Bacteria Lysis Buffer (Cat. No. 04 659 180 001):
200 µl of liquefied samples of mammalian origin (e.g., stool, urine, bronchoalveolar lavage [BAL], sputum, cerebrospinal fluid [CSF], swabs, or bacterial cultures)
