MegaMix Blue Double
MegaMix Blue Double: PCR-ready mix with Taq DNA polymerase, 400 μM dNTP, 5 mM MgCl2, and blue dye in Microzone’s buffer. Optimized for high yields, the blue dye aids visualization without interfering with downstream processes.
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Containing all the components needed to perform PCR swiftly and reliably. The 2X mix contains Taq DNA polymerase, 400 μM dNTP, 5 mM MgCl₂ and blue loading dye in Microzone’s proprietary enhancing buffer. MegaMix Blue Double is optimised to provide high yields under standard conditions. The blue agarose loading dye incorporated allows easy visualisation and eliminates the need for additional gel loading buffers. The dye does not inhibit restriction enzymes or ligases and does not fluoresce at the wavelengths used by automated DNA sequencers so downstream processes are not impacted.
Key Features
- More confidence in amplification and PCR test.
- Easy setup and PCR optimisation.
- Inert blue agarose loading dye allows for easy visualisation.
- Broad range of templates and conditions.
- Extremely stable—can be freeze-thawed many times.
- 2X concentrated to allow for increased sample volume.
Applications
- Routine PCR up to 6kb.
- Genotyping.
- TA cloning.
- Colony PCR.
- Amplification of GC and AT rich templates.
- Methylated DNA.
