MegaMix Gold
This 2X concentrated format can be used with a broad range of templates and conditions for end point and qPCR with addition of a dye such as MicroGREEN or single plex probe assays. Easy set up and PCR optimisation plus extremely stable with the ability to be freeze thawed many times.
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Containing all the components needed to perform PCR swiftly and reliably. The 2X mix contains Hot Start Taq DNA polymerase, 400 μM dNTP and 6 mM MgCl2 in Microzone’s proprietary enhancing buffer. MegaMix Gold uses a superior sensitive hot start DNA polymerase. The polymerase becomes active upon heating at 95°C. This ensures a highly specific and sensitive amplification, removing background and primer dimer formation. MegaMix gold boasts excellent accuracy and produces A-tailed products suitable for ligating into TA cloning vectors.
Key Features
- Hot Start polymerase in Microzone’s proprietary buffer gives unrivalled confidence in PCR amplifications.
- 2X concentrated format.
- A broad range of templates and conditions.
- Extremely stable—can be freeze thawed many times.
- Easy set up and PCR optimisation.
Applications
- Hot Start PCR up to 6 kb
- qPCR (probe or dye based)
- Fast PCR
- Multiplex PCR
- Genotyping
- Amplification of GC and AT rich templates
- TA Cloning
- High Resolution Melting
