MicroPlex Library Preparation™ Kit
The easiest NGS Library preparation you will ever find! The MicroPlex Library Preparation™ kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the True MicrochIP kit, it allows for performing ChIP-seq on as few as 10,000 cells. Studies showed a perfect match between the top 40% of peaks and the reference ChIP-…
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This was doubtlessly the easiest library prep kit I have used, needing only 1 day to make the library (while working on other projects as well!). I got good quality libraries when starting with 0.2-0.3ng DNA. The sequencing data have not been analyzed yet, and so I don't know how well the whole protocol worked, but for ease of use, this kit is great. It is a bit pricey, though.
Review Date: 27 Feb 2015 | Diagenode
The easiest NGS Library preparation you will ever find!
The MicroPlex Library Preparation™ kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the True MicrochIP kit, it allows for performing ChIP-seq on as few as 10,000 cells. Studies showed a perfect match between the top 40% of peaks and the reference ChIP-seq dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset. With less input, fewer steps, fewer supplies, and faster time to results, the MicroPlex kit is the library preparation solution of choice.
MicroPlex Library Preparation™ Kit Features:
- Simplified protocol: single tube, 3-step protocol without any intermediate purifications
- 3 times faster than TruSeq™ - only 2 hours for entire protocol
- DNA sample inputs from only 50 pg
- Specifically optimized for ChIP-seq
- Fewer PCR cycles: less bias
- Indexing options, easily automated
- Proven accuracy and reproducibility
Diagenode’s MicroPlex Library Preparation Kits v3 have been extensively validated for ChIP-seq samples and are optimized to generate DNA libraries with high molecular complexity from the lowest input amounts – down to 50 pg. The kit MicroPlex v3 includes all buffers and enzymes necessary for the library preparation. For flexibility of the choice different formats of compatible dual indexes are available separately.
