Mix-n-Stain™ CF® Dye Antibody Labeling Kits

Mix-n-Stain^™ CF^® Dye Antibody Labeling Kits allow you to label ≤5 ug up to 100 ug of your antibody in just 15 minutes, with minimal hands-on time and no purification.

Features

• Label 5-20 ug, 20-50 ug, or 50-100 ug Ab
• Choice of 32 CF^® Dye colors
• Less than 30 seconds of hands-on time
• 15 minutes total reaction time
• No purification, 100% recovery
• Compatible with BSA, gelatin, ascites

Kit components

• Ultrafiltration spin vial (for antibody concentration or buffer exchange, if needed)
• 10X Mix-n-Stain^™ Reaction Buffer
• Lyophilized Reactive CF^® Dye
• Mix-n-Stain^™ Storage Buffer

Unrivaled Convenience

Mix-n-Stain^™ labeling tolerates common antibody buffer formulations. An ultrafiltration centrifuge vial is provided to quickly remove interfering substances like glycerol, or to concentrate your antibody. With a slight modification in protocol, antibodies can be labeled in the presence of BSA, gelatin, or ascites fluid. Because CF^® Dyes are highly water soluble, the presence of other proteins like BSA or gelatin in the labeling reaction has minimal effect on background fluorescence, because any labeled non-antibody proteins readily wash away during immunofluorescence staining.

Superior CF^® Dyes

These kits feature a choice of 32 of Biotium’s next-generation CF^® Dyes, which were designed for advantages in brightness and photostability compared to Alexa Fluor^®, DyLight^®, and other fluorescent dyes.

Other Labeling Kits

Biotium also offers Mix-n-Stain^™ Maxi Antibody Labeling Kits for labeling 1 mg of antibody with CF^® Dyes, and Mix-n-Stain^™ Nanobody Labeling Kits. Mix-n-Stain^™ kits also are available for labeling antibodies with enzymes (HRP, AP, and GOx), fluorescent proteins (PE, APC, PerCP, and tandem dyes), biotin, or DIG.

Alexa Fluor and DyLight are registered trademarks of Thermo Fisher Scientific.