MolYsis™ Basic5

The vast amount of host DNA compared to microbial DNA in clinical samples often reduces the sensitivity in molecular analyses of microorganisms. MolYsis™ Basic5 kit is used prior to DNA isolation to deplete the host DNA and enrich the intact pathogen cells from small and large sample volumes. This leads to increased analytical sensitivity and can be combined with any commercial DNA extraction and isolation kits, manual or automated. With MolYsis™ Basic5 human or animal cells are selectively lysed and free DNA is degraded in a DNase treatment step. Afterwards a reagent, BugLysis, is used for the broad-range lyses of Gram-positive and Gram-negative bacteria and fungi. The enriched microbial DNA can be isolated and purified with the method of choice. Applications comprise next generation sequencing, amplicon cloning, real-time PCR, Sanger sequencing and DNA array analysis of microorganisms.

Benefits:

• Depletion of non-target, human or animal DNA
• Selective enrichment of bacteria and fungi
• Validated for a broad-range lysis of bacteria and fungi
• DNA-free reagents and consumables
• Suitable for fluids of 0.2-1 ml and 5 ml sample volumes
• Can be combined with any DNA isolation system of choice

The MolYsis™ technology is also available in the following products:

• MolYsis™ Complete5: host DNA depletion and microbial DNA isolation for fluid samples
• Ultra-Deep Microbiome Prep: as MolYsis™ Complete5 with additional protocol for tissues
• SepsiTest™-UMD CE IVD: all-in-one solution for clinical routine including manual microbial DNA enrichment & isolation and PCR assays
• MolYsis-SelectNA™plus: automated microbial DNA enrichment and isolation
• Micro-Dx™ CE IVD: all-in-one solution including automated microbial DNA enrichment & isolation and PCR assays