Monolith His-Tag Labeling Kit RED-tris-NTA
His-Tag specific labeling for MST using RED-tris-NTA
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Easy to use! Repeatable results!
Proteins
Easy to use and gives repeatable results!
Review Date: 26 Oct 2020 | NanoTemper Technologies
It has high affinity and this can be easy to use in biological direct binding assays.
MST - Microscale therapeutics (Monolith N9)
The Red-TRIS (NTA) dye is easy to use labeling kit for recombinant proteins with His-tag. It has high affinity and this can be easy to use in biological direct binding assays.
Review Date: 17 Jul 2019 | NanoTemper Technologies
Great tool. Highly recommended.
Biophysics
No protein loss with labeling. Very easy and straightforward to use.
Review Date: 8 Feb 2017 | NanoTemper Technologies
The Monolith NT His-Tag Labeling Kit is designed for MicroScale Thermophoresis (MST) applications, the method of choice for easy, rapid, and precise characterization of binding affinities.
The kit provides convenient means for the site-specific, purification-free labeling of small amounts of His-tagged proteins with our NT647 fluorescence dye. This kit can be used for the labeling of any protein or peptide carrying a polyhistidine-tag (His-tag) and contains everything required to perform labeling for 500 single-point MST measurements.
Labeling can be completed in 30 min, no removal of excess dye is required. The NT-647-His labeling dye binds efficiently to His-tags which contain at least six histidines with a Kd in the sub-nM range. By following this protocol, nearly a 100 % binding of dye to His-tagged proteins avoids any further purification step and ensures optimal results. The NT-647-His labeling dye shows fluorescence excitation and emission maxima at approximately 650 nm and 670 nm, respectively.
Features:
- Quick and easy: Mix-and-Measure approach
- No protein purification required - label straight in complex liquids like cell lysate
- Robust labeling in a variety of common storage buffers
- No purification steps required - no removal of free, unreacted dye
- Site-specific, stoichiometrically defined labeling - achieve a 1:1 ratio of labeled protein to dye
- MST optimized - the fluorescent dye is widely tested with MicroScale Thermophoresis (MST) and yields highly reproducible results
- Gentle to target biomolecule - non-covalent labeling
- Yields labeled protein sufficient for approx. 500 single-point MST experiments
- Label what you need - only minor amounts (picogram) of protein required
Applications:
- Any His-tagged protein
- Single-point screening
- Full affinity characterization
