Purification-Free Labeling in Whole Cell Lysate and Binding Characterization by MST

For researchers performing biophysical analysis of proteins, a common hurdle encountered is having sufficient amounts of materials with the appropriate purity to perform detailed analysis. Here we demonstrate the utility of a RED-tris-NTA dye from NanoTemper Technologies that can be used to specifically label His-tagged proteins for MicroScale Thermophoresis (MST) binding studies directly in cell lysates.