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Mycoplasma hominis IgG ELISA

DRG International Inc.EIA-4559Available: Worldwide

High Quality Assays with Reproducible and Reliable Results

DRG International Inc.

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An enzyme immunoassay for the qualitative and semiquantitative determination of IgG-class antibodies to Mycoplasma hominis in serum and plasma. The DRG Mycoplasma hominis IgG ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA). Microtiter wells as a solid phase are coated with Mycoplasma hominis surface antigen p120. Diluted patient specimens and ready-for-use controls are pipetted into these wells. During incubation Mycoplasma hominis-specific antibodies of positivespecimens and controls are bound to the immobilized antigens. After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgG antibodies are dispensed into the wells. During a second incubation this anti-IgG conjugate binds specifically to IgG antibodies resulting in the formation of enzyme-linked immune complexes. After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directlyproportional to the amount of Mycoplasma hominis-specific IgG antibody in the patient specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.

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