MyFi™ Mix

MyFi™ is a novel, antibody-mediated DNA polymerase complex with enhanced sensitivity and fidelity, ideally suited to problematic DNA templates.

MyFi Mix is a ready-to-use 2x mix formulation, containing all the components (including proprietary enhancers and stabilizers) necessary for trouble-free PCR reaction assembly, while delivering the same unique balance of PCR sensitivity and high-fidelity.

The pre-optimized MyFi Mix formulation, supplied in a convenient single tube, reduces the number of pipetting steps improving throughput and reproducibility.

MyFi Mix has been developed with an industry-leading, novel buffer chemistry containing pre-optimized concentrations of ultra-pure dNTPs, MgCl2 and proprietary PCR enhancers for exceptional versatility across a range of challenging assays including long PCR, low-copy PCR and amplification of problematic GC and AT-rich genomic templates. MyFi Mix generates extremely high DNA yields and produces 3'-A overhangs which are ideal for direct integration into TA-cloning vectors. In addition, MyFi Mix provides the convenience of room temperature reaction assembly and avoids non-specific amplification and primer-dimer formation.

Features:

• Convenient - ready-to-use, all-in-one 2x mastermix
• New generation DNA polymerase complex - provides robust PCR with 3.5-fold higher fidelity than wild-type Taq polymerase
• Antibody-based hot-start polymerase - enhances specificity and allows room-temperature reaction set-up
• High processivity - efficiently amplifies complex mammalian DNA fragments up to 10kb
• Novel 5x buffer system - ensures reliability, reproducibility and convenience

Applications:

• TA Cloning
• Long PCR
• Low-copy PCR assays
• PCR of difficult DNA templates (problematic GC/AT-rich genomic DNA)
• High-fidelity PCR assays (library amplification)