NEBNext® dsDNA Fragmentase®
NEBNext® dsDNA Fragmentase® generates dsDNA breaks in a time-dependent manner to yield 100–800 bp DNA fragments depending on reaction time. NEBNext dsDNA Fragmentase contains two enzymes, one randomly generates nicks on dsDNA and the other recognizes the nicked site and cuts the opposite DNA strand across from the nick, producing dsDNA breaks. The resulting DNA fragments contain short overhangs, 5´-phosphates, and 3´-hydroxyl…
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Simple and consistent.
DNA fragmentation
Really nice for the purpose that we wanted it for. The protocol is simple and straightforward, and the results are consistent. I am very happy with this product.
Review Date: 18 Jun 2021 | New England Biolabs Inc.
Great results, similar to acoustic shearing but easier!
DNA fragmentation and library preparation
High quality results, similar to more cumbersome acoustic shearing. I consulted NEB customer service for a custom application of this product, and they were helpful as always.
Review Date: 2 Oct 2019 | New England Biolabs Inc.
NEBNext® dsDNA Fragmentase® generates dsDNA breaks in a time-dependent manner to yield 100–800 bp DNA fragments depending on reaction time.
NEBNext dsDNA Fragmentase contains two enzymes, one randomly generates nicks on dsDNA and the other recognizes the nicked site and cuts the opposite DNA strand across from the nick, producing dsDNA breaks. The resulting DNA fragments contain short overhangs, 5´-phosphates, and 3´-hydroxyl groups.
NEBNext dsDNA Fragmentase does not introduce any detectable bias during the sequencing library preparation and no difference in sequence coverage is observed using the two methods
NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. The reagents included within these master mix sets must pass rigorous quality control standards and are lot controlled, both individually and as a set of reagents.
NEBNext® dsDNA Fragmentase® Includes:
- E. coli DNA Ligase for Fragmentase (10,000 units/ml)
- NEBNext® dsDNA Fragmentase Reaction BufferBAD (10X)
- BSA (10 mg/ml)
