NEBNext® Enzymatic Methyl-seq Conversion Module
NEBNext® Enzymatic Methyl-seq (EM-seq™) is a new method for identification of 5-mC and 5-hmC.
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Uniform and easy protocol.
Epigenetic methylation analyses
The NEBNext EMseq kit is a very good alternate to bisulfite sequencing to analyze the CpG methylated sites. I have been using this kit for mouse genomics DNA for methylation analyses. It's very simple and highly efficient compared to conventional bisulfite sequencing from our hand.
Review Date: 7 Jun 2021 | New England Biolabs Inc.
While bisulfite sequencing has been the gold standard for methylome analysis, this conversion treatment damages DNA, resulting in fragmentation, loss and bias. The NEBNext Enzymatic Methyl-seq Conversion Module provides an enzyme-based alternative to bisulfite conversion.
For use in Illumina® sequencing workflows, they recommend use of the NEBNext Enzymatic Methyl-seq Kit (NEB #E7120) instead of this module. The full kit includes the EM-seq Adaptor, which has been optimized for high performance in the EM-seq workflow, as well as the high-efficiency Ultra II library prep reagents, and these components are not included in the Conversion Module.
- High efficiency conversion
- Minimal DNA damage
- Minimized GC bias
- 5-mC and 5-hmC detection
- Complete kit including library prep reagents also available
