NEBNext® Reagents for DNA Library Preparation
NEBNext® reagents are a series of highly pure reagents that facilitate library preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. Available in sets, master mixes and modules, these robust reagents undergo stringent quality controls and functional validation, ensuring maximum yield, convenience and value. Advantages of NEBNext Reagents for RNA Library Pr…
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The NEBNext® End Repair Module and NEBNext® Q5® HotStart HiFi PCR Master Mix
Analyse DNA
NEBNext® DNA Library Prep Master Mix Set are excellent products we used to perform environmental DNA research on fish barcoding and multiplexing from our Partner NatureMetrics. Unfortunetly these products are difficults to purchase in LMIC.
Review Date: 31 Oct 2022 | New England Biolabs Inc.
The NEB Next products are easy and reliable methods for NGS library production.
NGS
I operate an NGS library preparation lab and the NEB Next suite of NGS kits work with and can adapt to just about anything I need to do. The customer service is also among the best when it comes to asking very specific application questions off-protocol. These products are my go-to for NGS library production.
Review Date: 27 Sept 2021 | New England Biolabs Inc.
cDNA synthesis
The NEBNext mRNA Second Strand Synthesis kit has proven superior to numerous others that we have tested in our lab. It is very fast and easy to use and at an incredible price for the purpose. Since discovering this method it has become a staple in our lab for cDNA synthesis. The technicians at NEB were the ones to originally point us in the direction of this method even before it was officially marketed and we are all very happy that they have helped us optimize our methods.
Review Date: 3 Jun 2013 | New England Biolabs Inc.
Molecular Biology
They are very easy to use.
Review Date: 5 Mar 2013 | New England Biolabs Inc.
NEBNext® reagents are a series of highly pure reagents that facilitate library preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. Available in sets, master mixes and modules, these robust reagents undergo stringent quality controls and functional validation, ensuring maximum yield, convenience and value.
Advantages of NEBNext Reagents for RNA Library Preparation:
Convenient formats – Enzymes, buffers and nucleotides are included, at appropriate concentrations and in appropriate volumes, and are available in set or master mix format. Large and small sizes are available.
Functional Validation – Each set is validated together by preparation of a library from an accepted RNA reference sample, that is sequenced using an Illumina GAIIx.
Stringent Quality Controls
Value Pricing
Novel Protocol for small RNA - Higher yield and substantially reduced adaptor dimer formation suitable for methylated and unmethylated small RNAs.
Applications of NEBNext Reagents for RNA Library Preparation:
mRNA library preparation for next generation sequencing
Small RNA library preparation for sequencing with Illumina and SOLiD
Expression library construction
