NEBNext® Ultra™ II FS DNA PCR-free Library Prep Kit for Illumina®
The NEBNext Ultra II FS DNA PCR-free Library Prep Kit for Illumina offers an amplification-free workflow for DNA-seq based on the streamlined and reliable Ultra II FS workflow. Starting with as little as 50 ng of DNA, get the high-quality, high-yield libraries that you need without PCR bias.
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Library preparation
It is easy to use, the product have two protocols, depending on your DNA input. the entire protocol takes less than seven hours.
Review Date: 26 Sept 2022 | New England Biolabs Inc.
The NEBNext Ultra II FS DNA PCR-free Library Prep Kit for Illumina offers an amplification-free workflow for DNA-seq based on the streamlined and reliable Ultra II FS workflow. Starting with as little as 50 ng of DNA, get the high-quality, high-yield libraries that you need without PCR bias.
- Includes NEBNext FS (Fragmentation System) enzymatic fragmentation reagents
- Perform fragmentation, end repair and dA-tailing with a single enzyme mix
- Save time with an optimized PCR-free workflow, reduced hands-on time and automation compatibility
- Generate high quality libraries without an amplification step using inputs from 50 ng to 500 ng intact DNA
- Benefit from improved library uniformity
- Use with the NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors DNA Set 1) or other Illumina-compatible index adaptors with 3′ single T overhang
