Ni-NTA Kit
The Gator ® Ni-NTA probes enabler rapid and continuous quantification of His-tagged proteins without the need for Ni2+ recharging. The stable immobilization of His-tagged proteins allows for facile kinetics analysis with binding partners, off-rate screening, and epitope binning of antibodies experiments.
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Gator® Ni-NTA probes are functionalized with Qiagen™ Tris-NTA and charged with Ni2+ ions for high affinity immobilization of His-tagged proteins. The probes can be used for quantitation of His-tagged proteins in sample buffer and cell culture supernatants. The specially formulated Gator® Ni-NTA Regen and Ni-NTA Neutral Buffers allow for continuous regeneration of the probes, without the need for an additional Ni2+ recharging step.