Nucleic Acids

To assist with RNA/DNA-related NMR- and/or MS-based research, CIL offers a large selection of various stable isotope-labeled compounds. NMR spectroscopy is an important tool to determine structures of nucleic acids alone and in interaction with proteins or small molecules. Nucleic acids are also increasingly being investigated in their use with potential biotherapies – RNA therapeutics being of particular interest. Nucleic acids containing roughly less than ~40 nucleotides will require simple 15N or 13C/15N enrichment to provide the constraints necessary to determine full three-dimensional structures. Advances in isotope-enrichment strategies have been crucial in the success for the study of larger RNAs. These advances primarily include the use of selectively and uniformly deuterated nucleotides and segmental isotope labeling using deuterium.