Octet® N1
Label-Free Analysis in a drop.
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Great instrument.
Biochemistry, immunology
It's a great product for evaluating kinetics and affinity for antibody-antigens interaction. Just need to provide a MacBook drive (only windows available).
Review Date: 2 Feb 2021 | Sartorius Group
simple and effective
RNA-binding proteins
The key advantage of the system is its easy of use, very straightforward, a student can be easily instructed to its use. It is an excellent resource for the determination of association and dissociation rates. On the downside, the lack of temperature control makes the reproducibility of the experiments dependent on the room temperature, which can be hard to control precisely. Also the software for analysis could be improved.
Review Date: 2 Aug 2017 | Sartorius Group
The Octet® N1 system is the perfect starter instrument for any lab, with an affordable price and almost instant BLI access. The portable benchtop system is designed for small experiments, its single-sample format makes assays easy to transport, set up and run.
Even if you're new to label-free assays, you'll be on your way to getting information-rich, real-time data in no time. The system provides BLI in a drop, requiring only 4 µL of sample, allowing protein quantitation and kinetics experiments when working with precious samples.
Key applications that leverage the unique strengths of system include monitoring concentrations of target proteins during cell line development and protein purification and confirming protein activity.
Key benefits:
- Stop waiting on results — check for the presence of proteins in media or lysate in 30 seconds
- Get way more than A280 — quantitate specific and active proteins in solution, not just total protein
- Optimize with purpose — get more insight into specific assay conditions for more robust ELISAs
- Troubleshoot immunoassays faster — check antibody binding and biotinylation to account for variation
- Watch analyte binding live — characterize protein interactions and determine rate constants at a fraction of the price of other methods
