ONE-Glo™ Luciferase Assay System
The ONE-Glo™ Luciferase Assay System provides a highly sensitive, robust, homogeneous assay for detection of firefly luciferase reporter gene expression in mammalian cells. Ideally suited for high- and ultrahigh-throughput applications, the ONE-Glo™ Assay contains a new luciferase substrate, resulting in a reagent that is more stable, more tolerant to sample components, and has less odor than standard luciferase assay reagents…
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Fantastic for detection and quantitation of reporter expression
Expression of reporter construct
By and large the best system for performing high throughput screening in the search for entities that alter expression (either basal or stimulated) of reporter constructs. Competing products don't even come close to the strength of signal or ease of use.
Review Date: 16 Nov 2021 | Promega Corp.
Great results.
Cell based reporter assay
High quality reproducible results. Protocol could be modified to decrease the time it takes to complete the assay and increase throughput.
Review Date: 27 Oct 2019 | Promega Corp.
Speedy and easy to use.
cell functional assay
It is very reliable for my luciferase assay. I always get reproducible data using this reagent. It is very straight forward to use. I am very impressed by the speed of their shipment, I always get the product next day.
Review Date: 25 Oct 2019 | Promega Corp.
We use this routinely and it is very easy to use with good reproducibility.
Review Date: 28 Jun 2011 | Promega Corp.
The ONE-Glo™ Luciferase Assay System provides a highly sensitive, robust, homogeneous assay for detection of firefly luciferase reporter gene expression in mammalian cells. Ideally suited for high- and ultrahigh-throughput applications, the ONE-Glo™ Assay contains a new luciferase substrate, resulting in a reagent that is more stable, more tolerant to sample components, and has less odor than standard luciferase assay reagents. These features ensure that the ONE-Glo™ Assay provides robust performance and also eliminates many of the handling inconveniences experienced using other reporter assays in a high-throughput setting.
Features - Benefits
- Simplify Your Assay Optimization: The robust performance, reduced odor, improved storage, and larger available sizes make assay optimization even easier and more efficient.
- Store at Room Temperature or at 4°C: The extended stability of the ONE-Glo™ Reagent at room temperature and 4°C makes it more convenient for everyday use.
- Improve Assay Precision: Because the ONE-Glo™ Reagent is less sensitive to mixing and dispensing conditions, reproducibility is enhanced. The assay is ideal for use in high-density (384- and 1536-well) microplates.
- Get a Brighter, Longer-Lasting Signal: Optimized for batch and continuous-process handling, the extended bright light output of the ONE-Glo™ Assay allows high sensitivity, especially when extended incubation is required prior to reading results.
- Reduce Unwanted Effects from Sample Components: The novel chemistry used in the ONE-Glo™ Assay is less sensitive to culture media, phenol red, and luciferase inhibitors than other luciferase assays.
